Field Portable Methods for Rapid Water Quality Analysis

Abstract

The availability of potable water in the field is essential to protect the health of soldiers in combat and training. Increasingly, the danger of coliform bacteria and other pathogens in water supplies has become a major concern. Conventional methods based on cell culture techniques for the detection of pathogens or their indicator organisms can require 24 hours or more to complete. Moreover, the detection and measurement techniques used are often complicated and not portable. Recent and on-going advances in gene amplification and related measurement technologies have permitted in this Phase II SBIR project the development of a potentially poffable process to determine E. coli bacteria in water in less than three hours and with a sensitivity of one bacterium. The individual components employed in the water characterization process can easily be combined into a portable package for field evaluation of water quality. Subsequent improvements in the technology are expected to permit a near on line measurement capability and to extend the capability to other pathogens in an easy-to-use portable field kit configuration.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1996
Accession Number
ADB229330

Entities

People

  • Larry J. Moore
  • Muralidhara Padigaru

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacteria
  • Chemical Reactions
  • Chemistry
  • Culture Techniques
  • Detection
  • Drinking Water
  • Environment
  • Environmental Protection
  • Escherichia Coli
  • Health Services
  • Medical Personnel
  • Microbiology
  • Microorganisms
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Thermal Cyclers
  • Water Purification

Fields of Study

  • Environmental science

Readers

  • Microbial Pathology
  • Nuclear Civil Defense.
  • Systems Analysis and Design