Sensitive, Specific Complementary - Strand Optical Detection of Viral RNA
Abstract
The feasibility of a novel low-cost sequence-specific nucleic acid detector of great speed and dynamic range has been examined and each system component has been tested to confidence. This detector records binding events at the surface of optical wave-guides, through coupling to the evanescent wave. The detector is made possible through innovative phase measurement techniques practiced by Visidyne. The laboratory design exceeds current detection noise threshholds and holds great promise Its components have been characterized and proven to show unparalleled sensitivity; in addition, an innovative method for improving sensitivity a thousand times is presented. The novel detection and bandpass filtering techniques exceed the state of art in evanescent-wave biosensing technology. Development of this system requires technical mastery in many disciplines; therefore, diverse concepts and data are discussed. The results suggest a likely strategy by which the system components, biochemical and electronic can be integrated to form a revolutionary new technique for chemical sensing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1997
- Accession Number
- ADB229334
Entities
People
- Ronald J. Rieder