Development and Validation of an Exogenous Metabolic Activation System for FETAX
Abstract
Frog Embryo Teratogenesis Assay: Xenopus (FETAX) is a 96-hour, whole-embryo bioassay designed to detect potential developmental toxicants. The primary goal of this research was to improve the predictability and increase the overall utility of FETAX as a screen for developmental toxicants that pose a hazard to human health. The first specific aim was to explore the use of phenobarbital, B-naphthoflavone, and isoniazid-induced rat liver microsomes as an exogenous metabolic activation system. This particular inducing system supplanted the presently-used Aroclor 1254 system which was recently proven to be somewhat unreliable. Post-isolation mixtures of these microsomes represented a broader spectrum of P-450 isozymes, and thus, bioactivated/inactivated a wider range of compounds. The second specific object was to develop and evaluate a standardized metabolic activation system test kit for FETAX which could be used by other FETAX users, including the U.S. Army. The findings from this research demonstrated that the post-isolation mixed microsomes were highly effective and that the test kits provided a reliable standardized means of routinely using the exogenous activation system with FETAX.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1997
- Accession Number
- ADB229442
Entities
People
- Douglas J. Fort