Detection of Arthropod-Borne Pathogens By Rapid Panel Assays in Mosquitoes
Abstract
The overall goal of Phase I of this project was to develop a rapid, one step, rugged immuno-chromatographic dip-stick assay for the identification of malaria and dengue-infected mosquitoes. This assay is based on the existing know-how at Navix by which sensitive test-strip based single analyte or panel assays provide results in less than 15 minutes and the tests are stable at ambient storage conditions. Phase I involved (1) Selection of the most appropriate antibodies for incorporation into the malaria and dengue assay that met the required criteria; (2) Development of prototype malaria assays with the required sensitivity; (3) Development of dengue assays with the required sensitivity and (4) Production of approximately 1000 assays of various malarial sporozoite antigens for laboratory /field evaluation. Tests supplied for evaluation with this report include those for the Plasmodium falciparum, Plasmodium vivax variant 210 and Plasmodium vivax variant 247 sporozoite antigens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1997
- Accession Number
- ADB229467
Entities
People
- Kirti I. Dave