Rapid Membrane Based Immunoassays for Detection of Toxins and Infectious Disease Pathogens
Abstract
Arista Biologicals, Inc. proposes to develop a series of assays to detect infectious disease pathogens and toxins on a series of rapid nitrocellulose membrane based assays utilizing colloidal gold and fluorescent dyes in both a unitized and multi-analyte format capable of semi-quantitation with a simple, inexpensive reader similar to a home diabetic glucose meter. We have formatted and initially tested 6 of the pathogens in the unitized format and have developed working tests for both dengue and hanta viruses. We plan to continue our investigation of the use of fluorescent dyes side by side with visible dyes, as the initial work has shown as at least comparable results and indicates that with a UV reader we may well find the enhanced sensitivity that we originally predicted. We have completed the exploratory work and initial testing of a reader system for enhanced readability and quantification and plan on continuing those efforts in the Phase II segment of the project. All of these efforts will enhance the detection capabilities of rapid assays for both military forward deployment and civilian point of care applications
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1997
- Accession Number
- ADB229914
Entities
People
- John Colanduoni