Study of Small Ligands Which Bind Specifically to Breast Cancer Cells
Abstract
Our lab is attempting to identify small ligands which bind specifically to breast tumor cells for ultimate use in tumor-targeted therapy. Our major target is the extracellular domain (ECD) of the ErbB2 growth factor receptor, which is overexpressed on the tumor cells of many breast cancer patients. This year we have succeeded in purifying ErbB2-ECD in the form of an ECD-alkaline phosphatase fusion protein to a high degree of purity and in amounts adequate to easily perform ErbB2-ECD affinity purifications and binding assays for putative ligands. This year we used a phage-displayed peptide library we constructed previously as a source of small ligands to ErbB2- ECD. We performed extensive screening experiments with our library against ErbB2-ECD target protein presented in different forms, bound to various matrices, and using several types of elution mechanisms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADB232935
Entities
People
- David N Krag
Organizations
- University of Vermont