Role of Human DNA Polymerase and Its Accessory Proteins in Breast Cancer
Abstract
Defects in DNA polymerase delta and its accessory proteins could contribute to the molecular etiology of breast tumors. In addition to a central role in DNA replication, recent work has shown that polymerase delta and its accessory proteins are also involved in DNA repair and that there are linkages between polymerase delta and cell cycle regulation via protein-protein interaction of polymerase processivity factor PCNA with p21, a cyclin dependent kinase inhibitor. Besides using immunoaffinity chromatography techniques to show that the deregulation of the cell cycle machinery in breast cancer cells, our preliminary data also show that the regulation of polymerase delta gene expression is different between normal breast cells and abnormal breast cancer cells. Results from the proposed studies could provide an understanding of the linkage between the regulation of polymerase delta and breast carcinogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1997
- Accession Number
- ADB233092
Entities
People
- Marietta Lee
Organizations
- University of Miami