Transgenic Rat Models for Breast Cancer Research
Abstract
The laboratory rat is an important model for studying breast cancer due to the many similarities in this disease between rats and humans. However, limited knowledge in manipulating the rat genome through transgenesis has prevented researchers from answering important questions in breast cancer research as well as in research on other human cancers. We proposed to carry out a series of detailed studies to optimize the many variables in transgenic manipulation, to extend transgenic rat technology to inbred rat strains, and to develop rat embryo cryopreservation. We have evaluated multiple variables in media composition and microinjection technique, generated multiple transgenic rat lines from several DNAs, all of which should be important for breast cancer research. We have further developed a procedure for rapid and efficient cryopreservation of rat embryos using morulae from superovulated rats that results in our ability to recover about half of frozen embryos as viable pups after transfer into pseudopregnant recipient rats. This procedure can be successfully applied to morulae obtained from rats of outbred Sprague-Dawley or inbred Wistar-Furth strains. In the course of establishing cryopreservation procedures, we have generated banks of frozen morulae from several transgenic rat lines and parental inbred strains that are used in breast cancer research.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1997
- Accession Number
- ADB235877
Entities
People
- Anne E. Griep
Organizations
- University of Wisconsin–Madison