The Role of Rearranged Neu Genes in the Progression of Rat Mammary Tumors Induced by N-nitroso N'-methylurea.
Abstract
Intramuscular injection of DNA expression vectors encoding the full length neu cDNA (pNeuN), the neu extracellular domain (pNeuE), or the neu extracellular and transmembrane domain (pNeuTM) can induce protective immunity in FVB/N mice against adoptive transfer of Tg1-1 cells, a neu over-expressing tumor cell line generated from a mouse mammary tumor that spontaneously arose in neu transgenic mice. Animal injected with control plasmid DNA could not resist tumor challenge. Anti-neu antibody response of mice immunized with neu expression plasmids were examined by Elisa assay, only some of the animals in pNeuN, pNeuTM, pNeuTM+pIL-2, or pNeuE+pIL-2 treated group were found to develop anti-neu antibodies, the animal in the other group did not develop a detectable anti-neu antibody response 30 days after DNA vaccination. This year, we examined anti-neu antibody response of DNA vaccine immunized mice again by indirectly flowcytometry assay, we confirm our previous finding. We also examine the cellular immunity response of DNA immunized mice by CTL assay, we find animal in DNA immunized mice did not develop detectable cytotoxic T lymphocyte specific against neu. Additionally, we have cloned and sequenced the mouse neu cDNA sequence from B6 mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1997
- Accession Number
- ADB236080
Entities
People
- Ying Chen
Organizations
- Salk Institute for Biological Studies