Hormonal Regulation of Hypothalamic Corticotropin Releasing Hormone.
Abstract
The expression of the endogenous Corticotropin Releasing Factor (CRF) gene was examined in the human neuroblastoma cell line, BE(2)-M17. In this cell line, treatment with retinoic acid induces CRF mRNA transcription. We examined the requirement for the POU transcription factor, Bm-2, for this response. We confirmed that Bm-2 is expressed in retinoic acid-induced BE(2)-M17 cells. Expression of antisense Bm-2 message aborted the retinoic acid induction of CRF transcription. However, over-expression of Bm-2 was not sufficient for CRF expression in the absence of retinoic acid. These experiments support the hypothesis that Bm-2 is an intermediary for retinoic acid-induced CRF expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADB236757
Entities
People
- Thiruvamoor P. Ramkumar
Organizations
- Washington University in St. Louis