Nitric Oxide in Mammary Tumor Progression

Abstract

Nitric Oxide (NO) is a potent bioactive molecule produced in the presence of endothelial (e), neuronal (n) or inducible (i) types of NO synthase (NOS) enzymes. We had earlier shown that treatment with NOS inhibitors reduced tumor growth and metastasis of C3H/HeJ murine mammary carcinomas, mitigated IL-2 therapy-induced capillary leakage and improved antitumor effects of IL-2. These results suggested that NO promoted mammary tumor progression and mediated IL-2 therapy-induced capillary leakage. Present proposal was to identify the underlying mechanisms for the above. Results to date reveal that eNOS expression by tumor cells is positively associated with metastasis in spontaneous C3H/HeJ mammary tumors and their clonal derivatives differing in their ability for spontaneous lung metastasis; that endogenous and induced NO promoted invasiveness of the highly metastatic C3L5 mammary tumor line owing to an upregulation of matrix-degrading enzyme matrix metalloprotease (MMP)-2 and downregulation of MMP inhibitors TIMP-2 and TIMP-3: that endogenous NO promoted C3L5 mammary tumor angiogenesis in a tumor-angiogenesis model devised in this laboratory. Thus NOS inhibitors may have a valuable role in cancer therapy.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1998
Accession Number
ADB238947

Entities

People

  • Peeyush K. Lala

Organizations

  • Western University

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DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Blood
  • Breast Cancer
  • Cancer
  • Cardiovascular Physiological Phenomena
  • Cardiovascular System
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Colon Cancer
  • Enzyme Inhibitors
  • Lymphocytes
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  • Peptide Growth Factors
  • Proteins

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  • Biology
  • Medicine

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  • Immunology and Pathology
  • Molecular Biology and Genetics
  • Oncology