Mechanisms of PCBS-Induced Breast Cancer
Abstract
Our research is structured to investigate the role of polychlorinated biphenyls (PCBs) in breast cancer induction. Progress is reported in several areas. 1) Higher chlorinated PCBs (#77 + 153) i. induce cytochromes P-450, ii. increase in GST activity, iii. decrease catalase activity, and iv. decrease GSH levels in the livers of treated animals. 2) High concentrations of PCBs were found to cause significant increases in lipid peroxidation and oxidative stress in the livers of treated rats. 3) Mammary tissue levels of oxided DNA bases suggest a differential response of oxidative stress in PCB-exposed rats. 4) Our measurements of antioxidant enyme activities in human breast tissue cytosols show enormous inter-individual variations in activities. 5) Structure-activity relationships for PCB metabolites have been described for superoxide production, DNA strand break activity, and cytotoxicity (in several systems including MCF-7 human breast cancer cells). 6) Preliminary studies have been undertaken to react PCB metabolites with DNA bases and to develop the appropriate HPLC conditions to isolate and characterize the reaction products.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADB240496
Entities
People
- Larry W. Robertson
Organizations
- University of Kentucky