Identification of Telomerase Components and Telomerase Regulating Factors in Yeast
Abstract
In the yeast S. cerevisiae, CDCl3 has been shown to be important for both telomere replication and maintenance of chromosome integrity. One model for Cdcl3p function is that it associates with telomeres as a single-stranded binding protein and facilitates complete replication of the telomeric DNA by protecting the chromosome end and regulating access of telomerase to the chromosome terminus. Additional genes required for telomere capping activity and length maintenance were characterized through examination of the relationship between genes required for repair of DNA double strand breaks, CDCl3, and genes encoding telomerase components. These data suggest that the Ku heterodimer, Cdcl3p, and telomerase participate in distinct pathways required for telomere function. To further explore the mechanism of telomere replication, I identified CDCl3 and EST2 interacting proteins via yeast two-hybrid screens. Characterization of the EST2 interacting proteins may reveal additional telomerase components or regulators. STNl, a high-copy suppressor of cdc13-1ts, was identified as a CDC13-interacting protein. Interestingly, the Stnip-Cdci3p two-hybrid interaction is abolished by the CdC13-2est mutation. Thus, the CdC13-2est allele may define a domain of interaction with Stn Ip that is required for positive regulation of telomere replication.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1998
- Accession Number
- ADB240500
Entities
People
- Constance I. Nugent
- V. Lundblad
Organizations
- Baylor College of Medicine