The Role of Autocrine-Paracrine Cascades in Breast Tumor Metastasis
Abstract
We had previously shown a very metastatic clone of NRK, RC-20, is non-invasive in an in vitro assay. These cells produce large quantities of an alpha-chemokine. It was proposed that the neutrophils the chemokine were attracting to the tumor could act as enablers in the metastatic process of these metastatic, non-invasive tumor cells. The aim of the project is to ascertain if metastatic breast tumor cell lines produce and release more alpha-chemokines than their non-metastatic counterparts. If this is so, these findings would be consistent with the hypothesis that tumor associated neutrophils can act as enablers in the metastatic process. Four human breast cell lines, two estrogen dependent, non-metastatic lines and two estrogen independent, metastatic lines were examined for their expression of IL-8. Conditioned media from these lines were analyzed for their IL-8 protein levels. The constitutive levels of IL-8 are not detectable in the non-metastatic lines, whereas both of the metastatic lines constitutively release measurable quantities of IL-8. In the presence of the inducing agents IL-1beta or TNFalpha, cytokines often found in a tumor's milieu, the non-metastatic lines produce low levels of IL-8, whereas the metastatic cells are very responsive and release high levels of IL-8.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADB240660
Entities
People
- Joseph E. Delarco
Organizations
- University of Iowa