The Role of SHP1 as a Tumor Suppressor Gene in Human Mammary
Abstract
To investigate the possible involvement of SHP1 in human mammary tumorigenesis, SHP1 was stably transfected into a human breast cancer cell line MDA-MB231, which has very low endogenous SHP1 level compared to normal breast epithelium. While the over-express on of SHP1 did not lead to any significant change in the proliferation rate of these cell it appeared to enhance their ability in anchorage-independent growth as well as their potential of tumorigenesis in nude mice. The over-expression of SHP1 changed neither the tyrosine phosphorylation status of overall cellular proteins or EGFR upon ECF stimulation in NDA-MB231 cells, nor the activation patterns of MAP kinases and AKT upon EGF and PDGF stimulation, respectively, in NIH3T3 cells. While more studies are necessary to further characterize the MDA-MB231 transfectants such as their invasiveness, it has also been planned to study the involvement of each important domain of SHP1 in tumorigenesis with a series of SHP1 mutants generated in this laboratory.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADB240902
Entities
People
- Chuan Gao
Organizations
- University of Texas at Austin