Catalytic Antibodies and DNA Site-Specific Recombination
Abstract
This is a report for the period from November, 1997 to September, 1998. The goal of this project is to develop catalytic antibodies that will allow efficient DNA transfer by promoting site-specific recombination. Such catalytic antibodies may provide a very powerful means to manipulate DNA transfer at defined sequences with obvious implications for gene therapy of breast cancer. In order to engineer enzymes that catalyze site-specific recombination, we have immunized mice and are continuing immunize mice with a synthetic Holliday structure. There are no positive clones identified so far, though we have screened over 1000 hybridoma clones. We have worked out the conditions for RT-PCR amplifying 6 pairs of light chains (out of 7) and all 9 pairs for heavy chains of antibody IgG sub 1 Fab variable region. Soon we will be able to construct a Fab expression library to screen for the Fabs that resolve the Holliday structure. Successful completion of this project will provide a new technology of DNA transfer for both biomedical research and therapeutic purposes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADB241963
Entities
People
- E. Aubrey Thompson
Organizations
- Baylor College of Medicine