Development of Intraductal Techniques for Breast Cancer Prevention, Diagnosis, and Treatment
Abstract
The purpose of this project was to develop a reliable, non invasive technique to gain access to the lining of the milk ducts. In a previous IDEA grant we demonstrated the feasibility of an intraductal approach to breast disease, the ability to obtain ductal cells through washings, and the general anatomy of the nipple duct orifices and ductal systems. In our first contract year, we demonstrated the ability to retrieve cells from women's ducts (pre and post mastectomy) through a newly developed double lumen catheter. After studying the microanatomy of the ductal orifices, we also began work on developing a technique for identifying the breast duct orifices. In this second contract year, we determined that our approach involving the dekeratinization of the nipple and application of a fluorescent keratin antibody to the ductal epithelium was not effective. A second approach was developed based on the random diffusion of a small molecular dye into the lactiferous sinuses of the ductal lumens. This method allows for the constant and reproducible identification of each and every duct. In addition, we have continued to refine the retrieval of duct cells in attached and detached breasts with refinements of the catheter as well as our techniques.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADB243030
Entities
People
- Susan Love
Organizations
- University of California, Los Angeles