Signaling Activity and Prognostic Significance of Variant Forms of p185HER-2/neu in Breast Cancer

Abstract

We investigated an N-terminally truncated HER-2/neu product of 95 kDa, for its relationship to shedding of the extracellular domain (ECD) of p185HER-2 and for its association with prognostic factors in human breast cancer. P95 had in vitro kinase activity indicating it is active as a signaling protein and its levels correlated with the ECD shed from different cells under varied conditions. Cancer tissues were analyzed by western blotting and scored for p95 and for p185HER-2/neu expression. Of 161 breast cancer tissues, 22.4% expressed p95, 21.7% overexpressed p185, and 14.3% were both p95 positive and overexpressed p185. A higher proportion of node positive patients (23 of 78) than node negative patients (9 of 63) expressed p95 in all tumors combined (P= .032).

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1998
Accession Number
ADB243181

Entities

People

  • Gail M. Clinton

Organizations

  • Oregon Health & Science University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Cellular Structures
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Cultured Cells
  • Diseases And Disorders
  • Governments
  • Lymph Nodes
  • Lymphatic System
  • Materials
  • Neoplasms
  • Oncology
  • Tissue Extracts

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics