A Mouse Model for the Cloning of a Tumor Suppressor Gene Mutated in Sporadic Breast Cancer

Abstract

Wnt/ wingless growth factors cause mammary tumors when overexpressed, and have many developmental functions. Yet despite extensive studies on the Wnt ligands, and the putative pathway components, little Is known about the reception mechanisms of signaling by these growth factors. A candidate Wnt receptor has been isolated using the yeast two-hybrid system. This protein has been shown to specifically bind four Wnt ligands. Truncated receptor forms, when misexpressed In Drosophila , lead to wingless loss of function phenotypes, strengthening its legitimacy as a Wnt receptor. The study's long-term objectives is to further characterize and functionally test this gene product. This will involve studies in both mouse and Drosophila, employing both ectopic expression studies and null mutation analysis. The proposed research should verify and elaborate the protein's role in Wnt / wingless signal transduction as it pertains to development and tumorigenesis.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1998
Accession Number
ADB244256

Entities

People

  • Martin J. Shea

Organizations

  • Baylor College of Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Animal Structures
  • Animals
  • Biomedical Research
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Diptera
  • Drosophila
  • Genes
  • Genetic Structures
  • Growth Factors
  • Hybrid Systems
  • Mammary Glands
  • Molecules
  • Neoplasms
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics