Identification and Characterization of Breast Tumor Associated Antigens
Abstract
Several aspects of breast cancer research, including detection, diagnostics, staging and immunotherapy strategies would benefit from identification of novel tumor antigens important in breast carcinoma. Because cancer patients exhibit both humoral and cellular immunity to tumor associated proteins, patient sera provides a valuable reagent for the identification of potentially immunogenic tumor associated antigens. We have used serological analysis of recombinant cDNA expression libraries (SEREX) for the identification of tumor antigens in a mouse model of adenocarcinoma and in human breast cancer. cDNA expression libraries derived from cell lines or tumor samples have been screened with autologous and allogeneic sera, and reactive clones have been identified. To date we have identified three candidate tumor antigens which may be important in human breast cancer. The clones encoding putative tumor antigens have been purified and are being characterized with regard to nucleic acid sequence, expression patterns, and reactivity with sera from allogeneic cancer patients. The longterm goal of this work is to develop these novel antigens as potential diagnostic and prognostic markers, and as targets for immunotherapy approaches to breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1998
- Accession Number
- ADB244279
Entities
People
- Theresa V. Strong
Organizations
- University of Alabama