Analysis of the Role of EST1 in Yeast Telomerase
Abstract
My research proposal focuses on characterizing the roles of proteins that are responsible for mediating access of telomerase to the telomere in the yeast S. cerevisiae. We have previously demonstrated that both Est1 and Cdc13 have biochemical and genetic properties that are consistent with those expected for proteins that mediate telomerase access. To define more clearly the mechanistic function of these two proteins at the telomere, I have pursued two experimental approaches over the past year. The first has been a detailed site directed mutational analysis of EST 1, with has yielded three particularly informative classes of mutants and has provided critical groundwork for the future Specific Aims of my research. In a second approach, I have examined the consequences of fusing Est1 to Cdc13, and have found that this fusion protein results in substantial telomere lengthening. The results of this second approach greatly support our model for the roles of Est1 and Cdc13 as co-mediators of telomerase access to the chromosome terminus. In addition, it has also provided us with a powerful tool to further genetically define regions of both of these two proteins involved in telomere maintenance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1999
- Accession Number
- ADB248925
Entities
People
- Sara K. Evans
- Victoria P. Lundblad
Organizations
- Baylor College of Medicine