The Role of the EGF Receptor and Vitamins A and D in Development and Progression of Breast Cancer to More Malignant Hormones Independent Phenotypes
Abstract
Epidermal growth factor receptor (EGFR) is a strong prognostic indicator for poor survival rate independent of estrogen receptor status, suggesting that EGFR overexpression is an important step in the progression to estrogen independence. The goal of this project is to understand how vitamins A and D regulate EGFR levels in hormone-dependent vs. hormone-independent breast cancer. Significant growth inhibition was seen in MCF7, T47D, and BT474 cells, but not BT549 cells, with 8 days 1,25-dihydroxyvitamin D3, analog C, 9-cis retinoic acid, or all-trans retinoic acid treatment. A 50% decrease in BT549 EGFR mRNA was observed within 2 hours of vitamin D treatment. After 3 days, vitamin D or retinoids resulted a 20-70% reduction in EGFR mRNA in MCF7, T47D, and BT549 cells, and a 200-500% increase in BT474 cells. EGFR protein levels correlated with these mRNA changes in BT474 and BT549 cells. Measurement of mRNA stability in vitamin D treated BT474 cells showed no change in EGFR mRNA half-life.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADB249407
Entities
People
- Susan A. Chrysogelos
Organizations
- Georgetown University