The Failure of Repair Enzymes in the Catechol Estrogen-Induced DNA Damage as Potential Initiating Event

Abstract

This report focuses on the ability of catechol estrogen-quinones to act as endogenous carcinogens, in that, they can cause DNA damage. Studies using Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) mass spectrometry indicated that estrogen-2,3-quinone can form stable adducts in 18 base oligonucleotides containing a single reactive site. Similar studies were carried out to demonstrate apurinic site formation in the same type of 18 base oligonucleotides after estrogen-3,4-quinone treatment. Since failure to repair or mis-repair is necessary for DNA damage to form a mutation, studies using apurinic site containing oligos were used to examine in vitro repair of apurinic sites. In addition, in vivo repair assays using apurinic-site containing pEGFP plasmids are, currently, being carried out.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1999
Accession Number
ADB251649

Entities

People

  • Kimberly A. Chapman

Organizations

  • University of Nebraska Medical Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cells
  • Chemistry
  • Electrophoresis
  • Estrogens
  • Gel Electrophoresis
  • Identification
  • Laboratory Animals
  • Mammary Glands
  • Mass Spectrometry
  • Materials
  • Molecular Dynamics
  • Neoplasms
  • Spectrometry
  • Tissue Extracts

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.

Technology Areas

  • Directed Energy