The Role of Autocrine-Paracrine Cascades in Breast Tumor Metastasis
Abstract
To determine if a correlation exists between the expression profile of interleukin-8 (IL-8), and the metastatic phenotype of breast tumor cells, the IL-8 levels produced by four breast tumor cell lines were assayed by ELISA. The constitutive levels of IL-8 were quantified for two metastatic and two non-metastatic human breast tumor cell lines. The data from these assays show the metastatic cells released much higher levels of IL-8 into their media than did the non-metastatic tumor cells. The higher levels of IL-8 mRNA produced by the metastatic tumor cells, as determined by RT-PCR, correlate with the amount of IL-8 released by these cells. A correlation was also observed between cellular morphology and IL-8 profile. Metastatic cell lines are more motile and fusiform in appearance, where the non-metastatic lines have a non-migratory, more epithelial appearance. These correlations suggest a possible enhancing or promoting role for IL-8 in the metastatic potential of breast tumor cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADB252910
Entities
People
- Joseph E. Delarco
Organizations
- University of Iowa