Identification of Telomerase Components and Telomerase Regulating Factors in Yeast
Abstract
In the yeast S. cerevisiae, the CDC13 gene has been implicated in both the telomerasemediated pathway for telomere replication and in maintaining chromosome integrity. One model for Cdc13p function at telomeres is that it functions as a single-stranded DNA binding protein and facilitates complete replication, thereby both protecting the chromosome end and potentially regulating access of telomerase to the chromosome terminus. Cdc13p, and its binding partner, Stnlp, may be critical targets for telomere length regulation, as alterations of either gene product can confer telomere shortening or lengthening phenotypes. I found that Cdc13p is phosphorylated in a cell-cycle dependent manner; the functional significance of the phosphorylation is not yet clear. Consistent with a role in DNA replication, Cdc13p appears to be enriched at telomeres during active DNA replication. However, Cdc13p may not function solely at telomeres, as it is also enriched at the centromere of chromosome III during S phase. A panel of mutant STN1 alleles should prove useful in dissecting its function as well as the nature of its interaction with Cdc13p.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADB253090
Entities
People
- Constance I. Nugent
- V. Lundblad
Organizations
- Baylor College of Medicine