Molecular Basis of Prostate-Specific Androgen-Independent Expression of a Homeobox Gene (Prostate)
Abstract
The goal of the work supported by this grant is to characterize the molecular basis of prostate specificity and androgen independence of the hoxb-13 gene with a view towards developing new treatments for advanced prostate cancer. To date, we have succeeded in generating a physical map of both the mouse and human hoxb-13 loci, and have sublconed regions both upstream and downstream of the coding region of both genes. Two reporter gene constructs carrying 4 or 8 kb of the human hoxb-13 upstream region have been completed and transgenic mice carrying these constructs have been generated. Analyses of reporter gene expression in these mice have revealed that the 8 kb fragment directs a pattern of gene expression that recapitulates features of hoxb-13 expression but does not direct expression in the prostate gland. Transgenes carrying regions farther upstream as well as downstream of hoxb-13 loci are being constructed to widen the search for cis-acting elements that function in the prostate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADB257204
Entities
People
- Charles J Bieberich
Organizations
- University of Maryland, Baltimore County