A Novel Repressor of Estrogen-Regulated Genes for Breast Cancer Growth Suppression

Abstract

The objective of this project is to develop and use novel ER mutants to test a two-part hypothesis. First, that the estrogen-independent growth of breast cancer cells involves the estrogen-independent expression of genes which are normally estrogen regulated. Second, that suppressing the expression of these genes will block the growth of breast cancer cells. To test these ideas, we set out to develop a novel type of estrogen receptor chimera which will efficiently and quantitatively suppress both estrogen-dependent and estrogen-independent expression of estrogen-regulated growth stimulatory genes. We used information from a genetic selection done using our modified P222 challenge phage system to identify mutations which lead to steroid receptor DNA binding domains with a strongly enhanced affinity for the estrogen response element (ERE).

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1999
Accession Number
ADB257305

Entities

People

  • David J. Shapiro

Organizations

  • University of Illinois Urbana–Champaign

Tags

DTIC Thesaurus Topics

  • Acids
  • Alkynes
  • Amino Acids
  • Anti-Bacterial Agents
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Coding
  • Enzyme Inhibitors
  • Governments
  • Illinois
  • Materials
  • Transcription Factors
  • Tumor Cell Line
  • Viruses

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics

Technology Areas

  • Biotechnology