Identification of a Modifier Locus Affecting Susceptibility to Mammary Tumor Development in Mice
Abstract
We have been using genetically predisposed mice to identify genes that can modify the risk of mammary tumor development. Apc(Min) female mice develop mammary tumors with a 5% incidence by 100 days of age. Treatment of female Mim/+ mice with ENU can increase the incidence of mammary tumors to greater than 80% within 65 days of treatment. We have characterized a congenic strain of ice that carries the ROSA26 insertion on chromosome 6, flanked by about 25 cM of DNA derived from the 129 strain. These mice are resistant to Min-induced mammary tumor development. In order to localize the locus or loci encoding the resistance, we have produced mice carrying subsets of the 129 derived DNA. Analysis of three such lines has indicated that a strong modifier maps within 2 cM of the ROSA26 insertion. We have identified further recombinant mice that should help to determine whether the modifier is separable from the ROSA26 insertion. We are also developing mice congenic or this region from the 129 strain that do not carry the ROSA26 insertion to allow us to determine whether the modifier is separable from ROSA26.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADB257443
Entities
People
- Amy R. Moser
Organizations
- University of Wisconsin–Madison