Genetic and Dynamic Analyses of Murine Peak Bone Density
Abstract
During our third year, the major effort has continued with genetic analyses of normal bone mineral density using inbred strains of mice differing by 35-50% in volumetric BMD. All (C57BUL/6x C3H/He)F2 and (C57BL/6x CAST/Ei)F2 mice have been phenotyped for femoral vBMD and (36 x C3H)F2 have been phenotyped for vertebral vBMD. Both F2 populations have been genotyped for the 10% of mice with the highest and 10% with the lowest bone densities. Genome-wide analyses of (B6x CAST)F2 mice revealed strong vBMD loci on 4 Chromosomes (Chrs 1, 5, 13, 15) and suggestive vBMD loci on 4 additional Chrs (3,4, 10, & 14). Genome wide analyses of (B6x C3H)F2 mice revealed strong vBMD loci on 5 Chrs (1,4, 11, 13, & 18) and suggestive vBMD 1oci on 3 additional Chrs (6, 14, & 16). Congenic strains carrying Chr regions from CAST (1,' 3, 5, 13, & 14) or regions from C3H (1, 4, 6, 13, &18) have significantly altered femoral vBMD as a consequence of the donated Chr segment. Preliminary mapping data for (B6x C3H)F2 vertebrae reveal 4 vBMD loci shared with femurs (1,4, 14, & 18) and 2 unique loci (Chr 7 & 9). Lastly, a new DEXA instrument for mice, the PIXImus, has been validated for body composition, and demonstrated capable of detecting the major loci for BMD shared with the femoral bone site.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADB257446
Entities
People
- Wesley G. Beamer