Patterns of Proteins That Associate With p53 or With p53 Binding Sites Present in the Ribosomal Gene Cluster and MDM2(P2) Promoter

Abstract

P53 is a tumor suppressor protein and its lack of function has been implicated in many-human cancers. To understand the molecular mechanisms underlying p53 function, will certainly provide tools to develop more effective therapeutical approaches against cancer. Our goal is to identify cellular proteins that associate with wild type p53 when p53 is bound to its binding site present in the internal promoter or the MDM2 gene (MDM2 P2) and when it is bound to its cognate site in the Ribosomal Gene Cluster. Using DNA affinity chromatography, we have found that the TATA binding protein (TBP) and two of its associated factors, TAFII40 and TAFII60, form a complex with wild-type p53 when p53 bids its cognate site in the MDM2 P2.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1999
Accession Number
ADB258240

Entities

People

  • Maria P. Molina

Organizations

  • Hunter College

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DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Carrier Proteins
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Fields of Study

  • Biology
  • Computer science

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  • Molecular Genetics
  • Plasma Physics.