A New Insulin-Like Growth Factor Binding Protein and Its Role in Breast Cancer and Cell Growth

Abstract

Mac25 is the first member of the IGFBP-rP family (IGFBP-rP1). IGFBP-rP1 is expressed in normal human mammary epithelial cells (HMECs) and upregulated in senescent HMECs. IGFBP-rP1 is not expressed in twelve/sixteen breast cancer cell lines and its expression is absent in estrogen receptor positive (ER+) breast cancer cell lines. IGFBP-rP1 may regulate HMEC growth. We tested the hypothesis that it suppresses growth in ER+ breast cancer cells by transducing the cDNA into normal and human breast tumor cultures. We are testing the function of IGFBP-rP1 in normal HMECs by transducing AG11132 normal cells. We will determine if IGFBP-rP1-rP1 is regulated at the transcriptional level by characterization of the promoter. We are determining the binding properties of IGFBP-rP1 by growth assays in presence of IGF-I, IGF-II, R3-IGF-I, and R6-IGF-II and immunoprecipitation/phosphatase assays. We have determined IGFBP-rP1 tissue specificity by mRNA in situ hybridization.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADB258646

Entities

People

  • Karen Swisshelm

Organizations

  • University of Washington

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Alcohols
  • Biological Sciences
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Epithelial Cells
  • Genetic Structures
  • Growth Factors
  • Hormones
  • Inhibition
  • Molecular Weight
  • Myoblasts
  • Neoplasms
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics