Cataloging Differentially Expressed Sequence Tags from Human Breast Cancer
Abstract
The purpose of the reported research was to identify novel genes whose differential pattern of expression could serve as useful diagnostic markers for human breast cancer. Using modifications of the differential display polymerase chain reaction technique, we successfully implemented a moderate-throughput method for isolating and directly sequencing novel cDNA fragments. An initial set of 150 cDNA fragments corresponded to mRNAs that were differentially expressed among five different human breast tumor cell lines. Sequence information from eight of these cDNAs was used to generate gene-specific primers. A semi-quantitative, multiplexed RT-PCR assay demonstrated that each of the eight genes had a unique pattern of expression among four, otherwise in-distinguishable primary human breast tumors. This work has resulted in methodology to isolate, catalog, and characterize sets of differentially expressed genes in microdissected, primary human breast tumors. A manuscript in preparation and subsequently awarded funding will continue This work toward the goal of developing novel breast, tumor markers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADB258750
Entities
People
- Mark Watson
Organizations
- Washington University in St. Louis