Functional Significance of Transcriptional Regulation by VEGF Receptor Tyrosine Kinases
Abstract
The relationship between angiogenesis and breast cancer tumorigenesis has been well established. VEGF is a leading candidate for a molecular mediator of tumor angiogenesis. VEGF is expressed by tumor cells in vitro and in vivo; blocking VEGF signaling arrests tumor angiogenesis and tumor growth in vivo. Based on these findings, it is clear that understanding the molecular mechanisms that drive the endothelial responses to VEGF will improve our understanding of tumor angiogenesis. Several of the signaling molecules that associate with the activated VEGF receptors influence pathways involved in transcriptional regulation in the nucleus. We hypothesize that the angiogenic effects of VEGF signaling are attributable, at least in part, to the activation of transcriptional regulation of gene expression. Thus, the goal of the study undertaken is to identify genes that are regulated by VEGF RTK activation and to determine the functional significance of these genes in tumor angiogenesis. We have completed two screens, a cDNA Representational Difference Analysis screen and another using the Gene Discovery Array filters. We further explored the role of C3G and CD9 in VEGF-mediated angiogenesis, but ruled them out. We are currently exploring the role of SOCS2, a negative regulatory protein, in the VEGF angiogenic pathway.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADB258877
Entities
People
- Adrianne L. Wong
Organizations
- Duke University Hospital