Prognostic Significance of Loss-of-Heterozygosity of the CUTL1 Putative Tumor Suppressor Gene in Breast Cancers
Abstract
We have defined a second region of LOH on the long arm of chromosome 7, within 7q22. 18% of spontaneous breast cancers were found to exhibit LOH of 7q22. The centromeric endpoint of the deletion was mapped to the CUTL1 gene, between introns 6 and 20. We found no correlation between LOH at 7q22 and tumor type or grade, ER or PR expression, nodal status, or age of onset. However, the average sizes of breast tumors with or without LOH at 7q22 were 4.99 and 2.93 cm respectively. We conclude that LOH of 7q22 will not have much prognostic value. We have determined the complete CUTL1 exon/intron structure, including the sequence at the 5' and 3' end of introns. The gene spans at least 340 Kbp and contains 33 exons. Synthesis of five different transcripts involves 2 promoter regions, 2 polyadenylation sites and 7 alternative splicing events. Analysis of CDP/Cut DNA binding activity during cell cycle progression and in breast tumor cell lines with various tumorigenic potentials indicated that CDP/Cut activity is associated with cellular proliferation. Thus, CUTLi is likely not the tumor suppressor gene on 7q22.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADB259959
Entities
People
- Alain Nepveu
Organizations
- McGill University