Development of a Transgenic Stem Cell Model of Prostate Cancer

Abstract

The overall goal of our proposal is to develop a new transgenic model of prostate cancer by targeting transformation to prostatic basal cells using the prostate stem cell antigen promoter (PSCA). A 9kb region upstream of the PSCA coding sequence was isolated and demonstrated to drive prostat-specific expression in vitro. This region was subsequently tested in vivo using a GFP reporter construct. Analysis of the PSCA promoter-GFP transgenic mice have demonstrated relatively prostate-specific expression of GFP. GFP expression in these mice is regulated developmentally, with prominent expression localized to the ductal tips of the prostate. Promoter activity appears to be androgen responsive and androgen independent. The PSCA promoter can drive target gene expression to the prostate of mice. Expression is restricted to a subset of prostatic cells, potentially the progenitor cells of the prostate. We are currently using this promoter to create new transgenic models of prostate cancer.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADB262090

Entities

People

  • Robert E. Reiter

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Androgens
  • Antigens
  • Biomedical Research
  • Cell Line
  • Cells
  • Computer Programs
  • Contractors
  • Gene Expression
  • Government Procurement
  • Governments
  • Materials
  • Neoplasms
  • Prostate Cancer
  • Sequences
  • Stem Cells
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech