Gene Discovery in Prostate Cancer: Functional Identification and Isolation of PAC-1, a Novel Tumor Suppressor Gene Within Chromosome 10p
Abstract
We have previously identified a novel genetic locus PAC-1 (Prostatic Adenocarcinoma-1) involved in tumor suppression of human prostate carcinoma. Introduction of human chromosome 10p into prostate adenocarcinoma cells resulted in tumor suppression and a restoration of the cell death pathway in vivo. The specific aims of this award involve both an expression based and positional cloning based strategy to identify PAC-1. As a first step in this strategy, we identified a region of homozygous deletion within a prostate cell line that falls within a region we identified as having tumor suppressor functional activity in vivo. Thus, we report the narrowing of the region containing PAC-1 to a homozygous deletion region. The next step in the strategy will be to clone the homozygous deletion region and identify this important tumor suppressor gene for prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADB262441
Entities
People
- Ann M. Killary
Organizations
- The University of Texas MD Anderson Cancer Center