Isolation of Genes Involved in Rac Induced Invasion and Metastasis of Breast Carcinoma Cells
Abstract
A better understanding of the progression of breast cancer to the metastatic state, and of the changes that take place in highly malignant breast cells at a molecular level, is essential to develop more direct forms of screening and therapy. Members of the RhoGTPases, in particular Rac, have recently been implicated in adhesion, motility and invasiveness, processes crucial for progression of breast cancer cells to the metastatic state. We have set out to identify target genes of Rac, as the screen may identify additional diagnostic markers, as well as provide new targets for therapy. To this end, we applied cDNA-RDA and microarray analyses to identify genes which are up- or downregulated as a result of Rac expression. These experiments resulted in the identification of 85 independent gene fragments (among them 23 novel genes), of which 37 were apparently upregulated and 48 downregulated. To ascertain the differential expression of the above genes, we initiated Northern blot analysis. Among the clones reconfirmed for difference in mRNA abundance are previously identified genes associated with tumorigenesis and genes whose predicted protein structure is suggestive for a role in invasion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADB262444
Entities
People
- Linda Van Aelst
Organizations
- Cold Spring Harbor Laboratory