Molecular Staging of Prostate-Bone Materials
Abstract
We have generated 81 individual clones of the LNCaP C4-2 sub line genetically tagged with bacterial Beta-galactosidase (BAG), and a separate C4-2-BAG-pool. The pool and 14 individual clones have been tested for PTHrP secretion in vitro and inoculated intra-cardially or subcutaneously into male SCID mice. BAG-PCR analysis showed a high degree of bone metastasis in 2 clones, medium in 4, low in 5, and none in 4. The BAG- pool, two high and three low bone-metastasizing clones are being subjected to repeat characterization in the SCID mice with intracardiac, subcutaneous, and intra-prostatic inoculation. In each case, material will be kept for histological analysis, ISH and IHC of PTHrP, and Laser Capture Microscopy (LCM). LCM has been developed for the human clinical material from radical prostatectomies, and clinical material, including primary prostate cancers, metastatic lymph nodes, associated seminal vesicles, and bone metastasis material, has been extended. We will ultimately perform gene array analysis on clones with differential bone metastasis potential, as well as at different stages in the mouse model, and examine the expression of candidate genes in the accrued clinical material.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADB262488
Entities
People
- Erik Thompson