Identification of Novel Prognostic Genetic Markers in Prostate Cancer
Abstract
Our progress on prostate cancer (PCa) to date has centered on the application of spectral karyotyping (SKY) and comparative genomic hybridization (CGH) to study consistent chromosomal changes. This work has resulted in three manuscripts (Appendices 1-3). We are now focusing on different cellular mechanisms that could lead to chromosomal aberration and consequent alterations of gene expression. To study early changes in the tumorigenic process, we have analyzed high-grade prostrate intraepithelial neoplasia (HPIN) using interphase flrorescence in situ hybridization (FISH). Our results suggest that the overall incidence of numerical abnormalities of chromosomes 7, 8 and 10 are more common in HPIN from patients who later showed carcinoma in a subsequent follow-up biopsy. Our recent results suggest that chromosomal instability (CIN) is a feature of Pca and that HPIN foci that progress to and/or are situated adjacent to carcinoma foci are more likely to express CIN than benign HPIN. To further study such early changes using molecular approaches we have developed degenerate oligonucleotide primed DOP-PCR methodologies to allow us to apply CGH methods using DNA microdissected from small foci of tumor or from PIN lesions at the time cancer arises in PCa patients. In parallel we are now studying alterations to gene expression and gene copy number in PCa using the most up-to-date CGH microarray methods.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2000
- Accession Number
- ADB262514
Entities
People
- Jeremy A. Squire