Puberty, Differentiation and Breast Cancer Risk

Abstract

Understanding molecular mechanisms of normal mammary gland development is essential for elucidating the relationship between normal, hormonally driven developmental events and carcinogenesis. We have studied a molecule, Brca2, which is highly expressed early in murine mammary gland development and report that this protein shares significant properties (upregulation at GI/S boundary, localization to "nuclear dots", interaction with Brcal) with its human homologue despite relatively low sequence identity (-57%). Additionally, we have undertaken a DNA microarray-based survey of gene expression during murine mammary gland development. Expression of 6000 genes has been ascertained at each of 13 developmental time points. We have developed informatics tools which allow us to manipulate and interpret patterns of gene expression within this data set, thus allowing us to look at mammary gland development in a more detailed way than has been possible heretofore.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADB262619

Entities

People

  • Lewis A Chodosh
  • Stephen R. Master

Organizations

  • University of Pennsylvania

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Computer Programs
  • Dna Microarrays
  • Epithelial Cells
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Mammary Glands
  • Microarray Analysis
  • Molecules
  • Neoplasms
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Systems Analysis and Design