Transcriptional Regulation of BRCA1

Abstract

Suppression of BRCAl transcription may contribute to the development of a subset of breast cancers. Previously. we described a positive regulatory region (PRR) in the BRCAl promoter (J. Biol. Cheni. 274,8837). Deletion of the PRR resulted in a significant loss of BRCAl promoter activity. In continuing studies we observed that the minimal BRCAl promoter extends from -202 to -156 (therefore excludes the start site). The promoter (with the PRR) primarily Consists of two discrete functional domains- (1) A polypyrimidinelpolypurine (Py/Pu) tract and (2) A CREB- (cAMP response element kinding) like binding site. Mutations in either of the domains severely reduced transcriptional and binding activity. Additional results strongly suggest that estrogen exerts its indirect, positive effect on BRCAl transcription through the PRR.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADB266113

Entities

People

  • Carlo Croce
  • Sanjay Thakur

Organizations

  • Thomas Jefferson University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Breast Cancer
  • Carrier Proteins
  • Cells
  • Chemical Reactions
  • Computer Programs
  • Contractors
  • Estrogens
  • Government Procurement
  • Governments
  • Maryland
  • Mutations
  • Neoplasms
  • Nucleotides
  • Polymerase Chain Reaction
  • Tumor Cell Line

Fields of Study

  • Biology

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