The Contributions of 8p Loss and 8p Gain to the Malignant Phenotype in Human Prostate Tumors

Abstract

The three specific aims of my project are: Specific Aim 1; transfect normal prostatic epithelium with the E6 and E7 genes of HPVl6 to produce immortalized cell lines, then genotype these cell lines to determine 8p and 8q status: retention of 8p sequences, loss of 8p sequences, or loss of 8p+gain of 8q sequences ISO(8Q); Specific Aim 2: determine whether loss of 8p sequences or loss of 8p+gain of 8q sequences is associated with expression of the transformed or invasive/metastatic phenotypes in the E6/E7 immortalized cells; Specific Aim 3 (Long Term Goals): isolate 8p-specific and 8q-specific genes that contribute to the transformed or invasivelmetastatic phenotype in E6/E7 immortalized cells.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2000
Accession Number
ADB266235

Entities

People

  • Rajiv Kant

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biology
  • Biomedical Research
  • Cell Line
  • Cells
  • Computer Programs
  • Contractors
  • Epithelial Cells
  • Genes
  • Genetic Phenomena
  • Genetics
  • Government Procurement
  • Governments
  • Neoplasms
  • Phenotypes
  • Prostate
  • Tissues

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics