Tagging of Breast Tumors for Excision and Specimen Radiography and of Sentinel Nodes for Ultrasound-Guided Localization Using Novel Particulate Agents
Abstract
Year 1, we identified a radiopaque tumor-marking agent and optimized the dose. This year we worked on coloring that agent and sentinel node detection. We could not color the radiopaque perfluorocarbon (PFC) emulsion, but successfully suspended India Ink within it. The optimum dose was then injected in tumors. We followed the black tract to locate/remove the tumor at 72-hours. The resected specimen was then radiographed. We worked on sentinel node detection with another agent. The black color identified the tumor in all rabbits. The radiopaque agent was visible within the tumor at 72-hours. Since it is difficult to color PFC emulsions, we abandoned the non-radiopaque PFC emulsion approach to locate the sentinel node. We identified a lipid emulsion where Sudan Black is dissolved within the vesicles. We have marked both the tumor with a radiopaque agent for specimen radiography and the tract to guide the surgeon to the tumor. We have identified a blue lipid emulsion that should have a longer dwell time in the lymphatics and a greater ability to localize in the sentinel node. The final year we will test the blue emulsion and compare its pharmacokinetics and sentinel node enhancement ability relative to the water-soluble blue dye.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADB266333
Entities
People
- Robert F. Mattrey
Organizations
- University of California, San Diego