Study of the Regulation of Telomere Replication by Characterizing the Cdc-13p Pathway in Yeast
Abstract
Telomeres are repeated double stranded DNA sequences at chromosome ends. Replication of telomeres requires telomerase. Recent data suggest telomere length maintenance might be involved in the origin or progression of cancer and aging. Understanding of telomere replication could offer new targets for drug screen. The conservation of telomerase and telomere structure has made yeast a good system to study the problem. An essential yeast telomere binding protein Cdcl3p appears to play a key regulatory role in telomere replication. Identifying Cdcl3p interacting proteins could yield important information. A two-hybrid assay was employed. Pollp, the catalytic subunit of DNA polymerase alpha(Pol alpha), and Est1p, a telomerase component, were identified. The two-hybrid interactions were also confirmed by biochemical criteria. Disrupting the Cdcl3p-Pollp interaction resulted in longer telomeres, while the mutated cells appeared normal in many aspects. The length of the elongated telomeres were in proportion to the extent of the decrease in the interaction. Data suggest that Pol a is involved in telomere replication, and Cdcl3p regulates telomere replication by interacting with both telomerase and Pol a. Other Cdcl3p interacting proteins were also identified. Further studies are in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADB266646
Entities
People
- Haiyan Qi
Organizations
- Princeton University