Identification and Characterization of Tumor Antigens Associated with Breast Cancer
Abstract
Recently, identification of tumor-associated antigens (TAAs) have led to an increased interest in vaccination strategies to treat cancer and/or prevent cancer relapse. Immunotherapy can be used as an adjuvant with other systemic therapy to target micro-metastatic disease and prevent cancer recurrence. Although preclinical/clinical trials using identified tumor-associated antigen for tumor vaccination are ongoing in melanoma and other cancers, breast cancer immunotherapy is limited, due to the limited numbers of breast tumor-associated antigens identified to date. Previously, three candidate breast tumor-associated antigens were identified in our lab by serological analysis of recombinant cDNA expression libraries (SEREX) derived from primary breast tumor and breast cancer cell lines (2-1-1, 7-3 and s3.2) . Initial characterization of these candidates by northern blot analysis revealed that 2-1-1 expression is tumor-specific while s3.2 clone is over-expressed in tumor. Although the expression of clone 7-3 is not restricted, its immunoreactivity is positive in high proportion of breast cancer patient sera (7/l4) . The evaluation of these putative tumor-antigens at the molecular level as well as further evaluation of their immunogenicity may provide insight in breast tumor biology as well as additional targets for breast cancer immunotherapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADB267108
Entities
People
- Alicia S. Racelis
- Theresa V. Strong
Organizations
- University of Alabama