Role of Seprase in Breast Cancer
Abstract
This is the final report documenting the achievements of DAMDI7-96-I-6097 "Role of seprase in breast cancer invasion". The goals were to produce full length seprase cDNAs and use these to produce cells that overexpress seprase. These seprase expressing cells were to be evaluated for invasive behavior relative to those that do not express seprase. The major goals of the project have been accomplished with the exception of engineering seprase- negative breast cancer cells to express active seprase. However, it was possible to evaluate the role of seprase in invasion by suppressing seprase expression in breast cancer cells that express seprase to a high level. Seprase suppressed transfectants of MDA-MB-436 and MDA-MB 435 breast cancer cells that express seprase were produced and compared to parental cells that express seprase in matrix proteolysis and invasion assays. These experiments suggested that seprase does not have a major role in promoting matrix degradation, invasion and metastasis. However, the experiments revealed that seprase has an important role in promoting breast cancer cell proliferation. These novel findings, described in this report, are the first to implicate seprase in breast cancer cell growth. The research has resulted in 3 papers, I manuscript, and 3 abstracts.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2001
- Accession Number
- ADB267657
Entities
People
- Thomas J. Kelly Jr.
Organizations
- University of Arkansas for Medical Sciences