Characterization of BRCA2 Transcriptional Regulation
Abstract
The purpose of this award is to study the transcriptional regulation of the BRCA2 breast cancer predisposition gene with the goal of identifying agents capable of modulating BRCA2 expression. Having previously mapped regions of the BRCA2 promoter that are involved in regulation of BRCA2 promoter function, we continued the project by examining the influence of a series of physiological and pharmacological agents on BRCA2 promoter function. Adriamycin, estrogen, serum starvation, forskolin, and tissue necrosis factor a either induced or repressed the promoter. In addition we demonstrated that the NFkB transcription factor can induce BRCA2 promoter function while adriamycin and p53 can repress the promoter. The observations that NFkB and p53 can regulate BRCA2 expression is expected to significantly impact our understanding of the pathways leading to cell death and to DNA damage repair. An improved understanding of the complex signals between the components of these pathways may facilitate design of novel therapeutic agents that can take advantage of these gene interactions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADB271165
Entities
People
- Fergus Couch