Volume-Stabilized Intravascular Microbubbles for Circulatory Transport of Oxygen and Carbon Dioxide: A Field-Usable Concept

Abstract

Intravascular microbubbles generated by i.v. infusion of a 2% dodecafluoropentane emulsion (DDFPe) transports physiologically significant amounts of oxygen in oxygen breathing rats (1). The present study explores the question whether DDFPe treatment can sustain life during air breathing in severely erythrocyte depleted pigs. Anesthetized pigs were bled while given volume replacement with 6% dextran in lactated Ringer's solution. Artificial ventilation and/or oxygen admixture (<4%) to the inspired air maintained PaO2 in the normoxic range (90-110 mm Hg) . Control animals (n=6) received emulsion blank in addition to the plasma expander. They died at a hemoglobin level of 3.0 g/100 ml. The experimental animals received 0.7 ml DDFPe/kg body weight in an i.v. infusion lasting for ^190 min of the 260 min long exsanguination period. These animals were observed for more than one hour at hemoglobin levels averaging 2.1 g/100 ml. They retained a normal PVO2 and acid-base status indicating adequate tissue oxygenation and arterial blood pressures remained well above shock level. We conclude that DDFP derived microbubbles hold promise as a very effective erythrocyte substitute for circulatory oxygen transport in situations such as combat casualty care. Hence, the treatment should be tested in a circulatory shock model.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2001
Accession Number
ADB274844

Entities

People

  • Claes Lundgren
  • Guri Bergoe
  • Ingvald Tyssebotn

Organizations

  • University at Buffalo

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Air Breathing
  • Blood
  • Blood Substitutes
  • Body Weight
  • Carbon Dioxide
  • Cardiovascular Physiological Phenomena
  • Casualties
  • Combat Casualty Care
  • Erythrocytes
  • Heart Rate
  • Hemorrhagic Shock
  • Laboratory Animals
  • Physiology
  • Respiration
  • Skeletal Muscle
  • Vehicles

Fields of Study

  • Biology

Readers

  • Marine Mammal Biology
  • Trauma Surgery or Emergency Medicine.