Regulation of C-myc Gene Expression by Potassium Channel Blocker Quinidine in MCF-7 Human Breast Cancer Cell Line
Abstract
C-myc is a protooncogene whose gene product has a regulatory role in cell cycle progression, cellular differentiation, and apoptosis. The abnormal expression of c-myc was reported in 32% of breast cancers, suggesting its importance in the genesis and/or progression of breast cancer. Therefore, it is important to understand the precise molecular mechanisms of c-myc regulation. The goal of this proposal is to elucidate how potassium channel blocking agent, quinidine, regulates expression of c-myc gene in breast cancer cells. Specific aim #1 was to test effects of quinidine on c-myc promoter activity (completed), identify quinidine responsive element (QRE) within c-myc promoter (completed), and study effects of quinidine on proteins/DNA interactions in the QRE (in progress). The aim #2 was to test effects of quinidine on c-myc transcription rate by nuclear run-on assay. In the current report the PI proposed to substitute this aim with the one that tests effects of quinidine on c-myc mRNA stability, due to the technical difficulties to detect small changes in c-myc transcription rate by run-on assay (not initiated). The significance of this proposal is that its results will improve our understanding of c-myc gene regulation and might lead to discovery of new targets for breast cancer therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADB277986
Entities
People
- Zaroui Melkoumian
Organizations
- West Virginia University