Characterization of a Novel Apoptosis Regulator BI-1
Abstract
BI-1 is an integral membrane protein that protects cells against Bax-induced cell death. It is commonly expressed in prostate cancer cell lines, making it a candidate regulator of prostate cancer cell death. I have used genetics, biochemical and cell biology approaches to understand how BI-1 regulates apoptosis. I have cloned BI-1 homologs from yeast, bacteria, Drosophila and plants, and demonstrated that they function similarly in yeast to protect Bax-induced cell death; I have discovered that the yeast BI-1 homolog is important in protecting cells against heat stress. I have also determined BI-1 domains essential for its function and proper sub-cellular targeting. BI-1 K/O mouse are also underway and characterization of these animals will reveal BI-1's in vivo functions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADB279621
Entities
People
- Ning Ke
Organizations
- Sanford Burnham Prebys Medical Discovery Institute